Differences in hematoxylin eosin (he) staining results on kidney, skin and colon histology of mice (mus musculus) based on cutting thickness of 3 μm, 6 μm and 9 μm)

Abstract

Influencing factors absorption coloring Hematoxylin Eosin (HE) is one of them size thickness cutting network, cuts are not appropriate and on time coloring that is not appropriate causes the absorption process color No perfect so that moment observation microscopic view of the cell nucleus and cytoplasm seen more pale and faint . Research objectives This is For know difference results coloring Hematoxylin Eosin (HE) on histology kidneys , skin , and colon mice (Mus musculus) based on thickness piece 3 µm, 6 µm, and 9 µm microtomes . Research methods This use method Experimental with nine groups treatment that is thickness cutting 3 μm , 6 μm and 9 μm microtome, then preparation done HE staining and observed quality microscopic includes the cell nucleus, cytoplasm and uniformity color. Data collection uses primary data, reading field done with 400x magnification (40x objective). Data processing uses statistical tests Mann Whitney . The results of the Mann Whitney test show the preparation kidney (p<0.05) that there is difference in a way significant , preparation skin cuts of 3 µm and 6 µm ( p > 0.05) that No There is difference significant cuts of 6 µm and 9 µm, 3 µm and 9 µm ( p< 0.05) that there is difference in a way significant, colon preparation ( p<0.05) that there is difference in a way significant. Conclusion of study This is in the kidneys cutting 3 µm microtome results preparation with quality the best coloring compared to with cutting 6 µm and 9 µm microtome , on skin and colon organs cutting 6 µm microtome results preparation with quality the best coloring compared to with cutting 3 µm and 9 µm microtome.